Screening Protocols
Comprehensive screening guidelines for STIs in patients with substance use disorder. Choose an infection for its protocol. Expand the guidance below for risk assessment and testing recommendations.
By infection
EHE Fast-Track HIV Referral
Rapidly transition newly diagnosed or out-of-care patients into a Ryan White Service Access Center within 24–48 hours.
Info link (Ending the HIV Epidemic)ADH Reporting & Contact Tracing
Arkansas Department of Health STD Prevention Portal for electronic laboratory reporting (ELR) and Disease Intervention Specialist (DIS) collaboration.
ADH portalCDC STI Clinical Practice Guidelines
The CDC's updated screening and treatment protocols — the authoritative quick-link for point-of-care reference.
CDC guidelines
Who to screen & the Five Ps
If a patient has had sex, has a new partner, has multiple partners, shares injection equipment, is pregnant, has symptoms, or thinks they've been exposed to an STI, talk with them about testing. Screening always begins with a sexual history — many STIs and HIV can be asymptomatic in people of any gender. Offer a sexual health assessment without assumptions; choose tests and intervals using infection-specific recommendations, age, anatomy, exposures, pregnancy, and local epidemiology.
To further guide your dialogue with your patient, the 5 "Ps" may be a useful way to remember the major aspects of a sexual history:
These are the areas to openly discuss with your patients. You'll likely need additional questions appropriate to each patient's situation — but the goal of the 5 Ps is to improve patient health, not simply to solicit full disclosure of sexual practices, especially if patients are not comfortable.
Several indicated STI tests can be collected at the same visit; there is no universal panel for every infection. Symptoms require diagnostic assessment. Bacterial vaginosis (BV) is a vaginal bacterial imbalance associated with sexual activity. Routine HSV serologic screening is not recommended, but type-specific HSV-2 testing has selected indications even without lesions.
Substance use & STI risk: the connection
Substance use raises STI risk through several pathways, not only injection. Understanding them helps target screening and counseling for patients with substance use disorder (SUD).
Clinical takeaway: screen patients with SUD more frequently, pair STI screening with a substance use screen, and treat both without judgment. Use the SBIRT framework below to structure that conversation.
SBIRT: screening & brief intervention for substance use
SBIRT (Screening, Brief Intervention, and Referral to Treatment) is an evidence-based approach for identifying and addressing substance use in any care setting: primary care, emergency departments, and SUD or STI programs. It pairs naturally with the sexual-history screening above.
Treatment Protocols
First-line treatment regimens for common STIs in SUD populations, consistent with current CDC STI Treatment Guidelines. Always confirm allergies and drug interactions before prescribing.
By infection
Where can I get the medicine? (patient assistance & access)
Cost should not be a barrier to treatment. Many STI/HIV/HCV medications are available at low or no cost through manufacturer patient-assistance programs (PAPs), 340B pricing at qualifying clinics, and state programs — often including people who are uninsured.
- Gilead Advancing Access — HIV/HCV/PrEP patient assistance
- ViiVConnect — ViiV Healthcare patient assistance (Apretude, etc.)
- NeedyMeds — search patient-assistance programs by drug
- Arkansas Department of Health — Ryan White Program / ADAP
- FindTreatment.gov — SAMHSA substance use treatment locator
- SAMHSA National Helpline — 1-800-662-HELP (4357), free, confidential, 24/7
Full treatment guidelines
The regimens below summarize first-line therapy. Always confirm current dosing, alternatives, and pediatric/pregnancy considerations against the source guidelines.
Quick treatment summary — all STIs
A plain-language overview, including infections managed rather than cured. Always confirm against CDC guidance before prescribing.
| Curablea full course of medicine clears the infection | |
|---|---|
| Chlamydia | Treated with antibiotics — usually cured with medicine. |
| Gonorrhea | Treated with antibiotics — usually cured with medicine. |
| Syphilis | Treated with antibiotics — early treatment can cure the infection. |
| Trichomoniasis | Treated with antibiotics — usually cured with medicine. |
| Hepatitis C | Treated with medication (DAAs) — most people can be cured. |
| Managed, not curedtreatment controls the infection long-term | |
| HIV | Treated with daily medication (ART) — helps people live long, healthy lives. |
| Herpes (HSV) | Treated with antiviral medication — reduces outbreaks and symptoms (not curable). |
| HPV | No cure for the virus; treatments are available for warts and related health problems. Vaccination prevents most high-risk types. |
| Hepatitis B | Some people recover without treatment; others need medication to manage the infection. Vaccine-preventable. |
Testing & Diagnostics
When to test, which tests to use, and how to collect and handle specimens — plus Arkansas testing and referral resources.
Recommended Tests
TEST NAME
Nucleic Acid Amplification Test (NAAT)
SAMPLE TYPE
- Male: First-catch urine (15-30 mL)
- Female: Vaginal swab (self or provider collected) or endocervical swab
- Extragenital: Rectal or pharyngeal swab
TURNAROUND TIME
2-5 business days
NOTES
CDC referenceCDC — Chlamydia
TEST NAME
Nucleic Acid Amplification Test (NAAT)
SAMPLE TYPE
- Male: First-catch urine or urethral swab
- Female: Vaginal or endocervical swab
- Extragenital: Rectal or pharyngeal swab (especially for MSM)
TURNAROUND TIME
2-5 business days
NOTES
CDC referenceCDC — Gonorrhea (adults)
TEST NAME
Screening: Treponemal (TP-PA, FTA-ABS) or Nontreponemal (RPR, VDRL)
Confirmatory: Opposite test type + quantitative titer
SAMPLE TYPE
- Serology: Venous blood (serum or plasma)
- Lesion: Darkfield microscopy or PCR from ulcer exudate (if available)
TURNAROUND TIME
2-5 business days
NOTES
CDC referenceCDC — Primary & Secondary Syphilis
ArkansasARPQC — Congenital Syphilis Prevention · ADH — Syphilis in Pregnancy & Congenital Syphilis
TEST NAME
4th Generation Antigen/Antibody Combination Immunoassay
Confirmatory: HIV-1/HIV-2 Differentiation Immunoassay + HIV-1 RNA if needed
SAMPLE TYPE
- Laboratory: Venous blood (serum or plasma)
- Rapid test: Fingerstick blood or oral fluid
TURNAROUND TIME
Laboratory: 2-5 business days
Rapid test: 10-20 minutes
NOTES
TEST NAME
Screening: HCV Antibody (anti-HCV)
Confirmatory: HCV RNA (quantitative viral load)
Additional: Genotype, liver function tests, fibrosis assessment
SAMPLE TYPE
- Serology: Venous blood (serum or plasma)
- RNA: Venous blood (plasma or serum)
TURNAROUND TIME
Antibody: 2-5 business days
RNA: 3-7 business days
NOTES
ReferenceAASLD/IDSA — hcvguidelines.org
TEST NAME
Symptomatic: Viral culture or PCR from lesion
Asymptomatic: Type-specific HSV-1/HSV-2 serology (if clinically indicated)
SAMPLE TYPE
- Lesion: Swab from vesicle fluid or ulcer base
- Serology: Venous blood
TURNAROUND TIME
PCR: 2-5 business days
Culture: 3-7 business days
Serology: 2-5 business days
NOTES
CDC referenceCDC — Genital Herpes (HSV)
How STI Testing Works
STIs are tested by urine sample, blood sample, swab, or saliva, depending on the infection and test type. Available tests vary by clinic. HIV, hepatitis C, and syphilis may have rapid options; gonorrhea, chlamydia, and trichomoniasis use assay-appropriate urine or swab specimens.
TURNAROUND
- Rapid tests: results may be available during the visit; timing depends on the assay
- Laboratory tests: often several days; some point-of-care GC/CT tests return results during the visit
CONFIRMATORY TESTING
- Reactive HIV/hepatitis C rapid tests need a confirmatory blood draw
- Syphilis: titer (RPR) (a blood test) — look for a fourfold decline over 6–24 months; in a previously cured, asymptomatic patient a titer that plateaus at a low level (≤1:8) is medically acceptable (serofast)
- Previously treated syphilis/hepatitis C may stay antibody-reactive — use RNA (hepatitis C) or titer (syphilis)
Nuances & Common Pitfalls
False positives
- Suspect a false positive when the result doesn't match the clinical picture or sexual history (more common with autoimmune conditions).
- Confirmatory testing, a thorough sexual history, and counseling are advised before acting on an unexpected result.
- Counsel every patient on prevention regardless of whether the result is a true or false positive.
Rural & resource-limited settings
- Where diagnostic testing isn't readily available, consider the syndromic approach — classifying and treating based on symptoms and signs rather than lab confirmation.
- Reference: PAHO — Syndromic Management of STIs
Common clinician errors to avoid
- Inadequate sexual-history taking, personal bias, misinterpreting the window period, and overlooking concurrent screenings.
- Use the CDC’s Five Ps framework on every sexually active patient. CDC — Taking a Sexual History
When to Test
AT PROGRAM INTAKE
- All individuals entering SUD treatment program
- Review prior testing; offer indicated STI, HIV, and hepatitis C screening
- Document sexual health history and risk factors
- Discuss pregnancy possibility and offer a pregnancy test when indicated
PERIODIC RESCREENING
- Use infection-specific intervals and reassess after a new exposure
- At least annual HIV testing with ongoing risk; at least annual HCV testing with ongoing injection drug use
- 3 months post-treatment for bacterial STIs (chlamydia, gonorrhea)
- Pregnancy: infection-specific schedule; syphilis at first visit, third trimester, and birth per ACOG/ARPQC
Arkansas testing & referral resources
Free and confidential testing is available at local health units. Use these Arkansas-specific resources for congenital syphilis prevention, syphilis case management, and client referral.
- ADH Local Health Units — free & private testing
- ARPQC — Congenital Syphilis Prevention
- ADH — Syphilis in Pregnancy & Congenital Syphilis
- Contacts for Reporting & Managing Syphilis Cases in AR (PDF)
- FindTreatment.gov — SAMHSA substance use treatment locator
- SAMHSA National Helpline — 1-800-662-HELP (4357), free, confidential, 24/7
High-Risk Population Indicators
Injection Drug Use
Current or recent history of injection drug use increases risk for HIV, Hepatitis C, and bacterial STIs.
Use infection-specific intervalsMultiple Partners
More than one sexual partner in past 3-6 months, or partner with multiple concurrent partners.
Use infection-specific intervalsPregnancy
All pregnant individuals require comprehensive STI screening to prevent congenital infections.
Follow pregnancy guidance by infectionInconsistent Condom Use
Condomless sex with partners of unknown STI status or known risk factors.
Use infection-specific intervalsMSM
Men who have sex with men have elevated risk for HIV, syphilis, and extragenital gonorrhea/chlamydia.
Assess current exposure and testing historyPrior STI Diagnosis (lifetime)
A prior STI prompts a review of recommended post-treatment retesting and current exposures. A lifetime history alone does not establish a three-month schedule.
Assess current exposure and testing historyLab & Specimen Guidance
Specimen Collection Best Practices
URINE COLLECTION
- First-catch urine: Collect first 15-30 mL of voided urine stream (not midstream)
- Timing: Patient should not have urinated for at least 1-2 hours prior to collection
- Container: Use sterile urine collection cup provided by laboratory
- Storage: Refrigerate if not processed within 2 hours; stable for 24 hours refrigerated
VAGINAL SWAB COLLECTION
- Self-collection: Patient inserts swab 2 inches into vagina, rotates 10-30 seconds, removes without touching skin
- Provider-collection: Visualize cervix with speculum, swab vaginal walls or endocervix
- Swab type: Use manufacturer-provided swab (typically Dacron or rayon, not cotton)
- Note: Self-collected vaginal swabs have equivalent sensitivity to provider-collected for NAAT testing
EXTRAGENITAL SWAB COLLECTION (RECTAL, PHARYNGEAL)
- Rectal: Insert swab 2-3 cm into anal canal, rotate gently for 10-30 seconds to sample crypts
- Pharyngeal: Swab posterior pharynx and tonsillar crypts bilaterally, avoid tongue and buccal mucosa
- Important: Verify laboratory accepts extragenital specimens for NAAT testing before collection
BLOOD COLLECTION (SEROLOGY)
- Volume: Typically 5-10 mL venous blood in serum separator tube (SST) or plasma tube
- Processing: Allow to clot (SST) or centrifuge (plasma), separate serum/plasma within 2 hours
- Storage: Refrigerate if not processed immediately, stable 7 days refrigerated
LESION SWAB COLLECTION (HSV, SYPHILIS)
- HSV: Unroof vesicle if present, swab base of lesion or ulcer vigorously to collect cellular material
- Timing: Collect within 48 hours of lesion onset for best yield (viral load highest early)
- Transport: Place swab in viral transport medium immediately, keep refrigerated or on ice
Specimen Handling & Transport
Labeling Requirements
- Patient name and date of birth
- Unique patient identifier or medical record number
- Date and time of collection
- Specimen source (e.g., urine, vaginal swab, rectal swab)
- Collector initials
Storage & Temperature
- NAAT specimens: Follow the specific assay’s collection kit, temperature, and transport-time requirements
- Viral culture: Use the laboratory’s required transport medium and handling instructions
- Serology: Follow the laboratory’s separation, storage, and transport instructions
- Freezing: Requirements vary by assay and specimen; some NAAT specimens may be frozen. Check the manufacturer’s instructions and receiving laboratory before storage
Packaging
- Place specimens in leak-proof primary container
- Use absorbent material between primary container and outer packaging
- Include laboratory requisition form in separate compartment
- Mark outer package as "Diagnostic Specimen" if required
Transport Timing
- Same-day preferred: Transport specimens to laboratory same day of collection
- Weekend collection: Confirm an acceptable collection, storage, and pickup plan with the laboratory first
- Viral culture: Follow the laboratory’s transport-time and temperature requirements
- Coordinate with laboratory for pickup schedule and requirements
Prevention, Partner Services & Data
Vaccination guidance, partner notification, and Arkansas surveillance data to support prevention in SUD treatment settings.
Vaccination Recommendations
| Vaccine | Who & when |
|---|---|
| HPV | Routine through age 26; may offer to age 45 via shared decision-making. Prevents most cervical and other HPV-related cancers. |
| Hepatitis B | Universal for adults 19–59; 60+ with risk factors. Strongly recommended for people who inject drugs. |
| Hepatitis A | Recommended for people who inject drugs and others at risk. |
| Mpox | Offer to eligible at-risk patients per current ADH guidance. |
Partner Services & Notification
Partner management depends on the infection: chlamydia/gonorrhea generally use a 60-day window, including the most recent partner if earlier. Syphilis uses stage-specific windows: primary, 3 months plus symptom duration; secondary, 6 months plus symptom duration; early latent, 1 year. Follow CDC syphilis guidance for evaluation and presumptive treatment. Expedited Partner Therapy (EPT) is permitted in Arkansas for gonorrhea and chlamydia. The state health department offers confidential partner notification — partners can be notified without revealing the index patient’s identity.
Arkansas STI Data & Surveillance
Arkansas has consistently reported STI rates above the national average, and congenital syphilis has risen sharply in recent years — mirroring a national increase reported by the CDC. Confirm current figures against the ADH and CDC data linked below, and use state surveillance data to target prevention efforts.
Reporting Requirements
STIs are reportable conditions in Arkansas. Submit case reports to the Arkansas Department of Health within the required timeframe to support partner services and surveillance.
- Communicable Disease Reporting Form (PDF)
- ADH HIV/STI Surveillance — adult and pediatric HIV confidential case report forms (in addition to the communicable disease form)
- Reportable Diseases List & Instructions (PDF)
- AR State Board of Health Rules Pertaining to Reportable Diseases, 2023 (PDF)
- Arkansas Code 20 CAR § 102-122 (statute)